Anti-Iba1 Mouse mAb
Product Information
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Protein full name |
Allograft inflammatory factor 1 |
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Synonyms |
AIF-1, IBA, IRT-1, IRT1, allograft, AIF-1, AIF1, G1, IBA1, IRT 1, Protein G1, Ionized calcium-binding adapter molecule 1, MRF1, Microglia response factor, BART 1, AIF1 protein |
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Immunogen |
Recombinant protein corresponding to Mouse Iba1 |
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Uniprot ID |
O70200 |
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Isotype |
IgG1,κ |
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Purity |
Affinity purification |
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Subcellular location |
Cytoskeleton, Ruffle membrane, Phagocytic cup |
Applications
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IHC(PFA fixed)/IF(PFA fixed) |
Mouse, Rat, Monkey |
1: 500-1: 4000 |
brain |
Background
AIF1, also named as G1, IBA1 or daintain/AIF-1, is a 143 amino acid cytoplasmic, inflammation response scaffold protein. It is constitutively expressed in monocytes and macrophages and is known to be involved in macrophage activation. It is a marker of activated macrophage. Despite a lack of detailed knowledge on the in vivo physiological functions of AIF-1, there is growing evidence that shows its aberrant expression contributes to the pathogenesis of many autoimmune diseases, including rheumatoid arthritis. AIF1 is an actin-binding protein that enhances membrane ruffling and RAC activation. It enhances lymphocyte migration and the actin-bundling activity of LCP1.
Images
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IHC analysis of IBA-1 (GB12105). |
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IHC analysis of IBA-1 (GB12105). |
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Immunohistochemistry of paraffin embedded rat brain using IBA-1 (GB12105) at dilution of 1: 2000 (300x lens) |
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Immunofluorescent analysis of paraffin embedded rat hippocampus using Neun (GB11138) (green) + Iba1 (GB12105) (red) + MBP (GB12226) (spred) + GFAP (GB11096) (yellow) and Dapi in blue at dilution of 1: 1000 |
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Immunofluorescence of paraffin embedded mouse brain using IBA-1 (GB12105) at dilution of 1: 1000 (200x lens) |
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Immunofluorescent analysis of paraffin embedded rat brain using Neun (GB11138) (green)+ Iba1 (GB12105) (spred) + MAP2 (GB11128-2) (red) and Dapi in blue at dilution of 1: 1000 |
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IF analysis of IBA-1 (GB12105). |
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IHC analysis of IBA-1 (GB12105). Sample: Human glioblastoma multiforme, 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH 9.0) (G1203),98℃,20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG(H+L), Ready to use(G1302), RT, 20min. |
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IHC analysis of IBA-1 (GB12105). Sample: Human oligodendrocytoma , 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH 9.0) (G1203),98℃,20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG(H+L), Ready to use(G1302), RT, 20min. |
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IHC analysis of IBA-1 (GB12105). Sample: Monkey brain, 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH 9.0) (G1203),98℃,20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG(H+L), Ready to use(G1302), RT, 20min. |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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