Recombinant Anti-beta Actin antibody (HRP Conjugated)
Product Information
|
Protein full name |
Actin, cytoplasmic 1 |
|
Synonyms |
ACTB, BRWS1, PS1TP5BP1, Beta-actin, actin beta, Beta Cytoskeletal Actin, β-Actin |
|
Conjugate |
HRP |
|
Purity |
Affinity purification |
|
Uniprot ID |
P60710 |
|
Predicted MW. / Observed MW. |
42 kDa / 42 kDa |
Applications
|
WB |
Human, Mouse, Rat |
1:1000-1:3000 |
HeLa, HepG2, brain, etc. |
|
Incubation condition |
After blocking, incubate the membrane with HRP conjugated anti-beta Actin antibody (ZB15001-HRP) for 2-3 hours at room temperature (20-25℃) or overnight at 4°C, and then wash the membrane 3 times for 5 mins each with TBST. |
||
Background
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.
Images
|
|
Westerin blot analysis of beta actin (ZB15001-HRP)
Sample: Protein treated by RIPA Lysis Buffer (G2002). |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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