Recombinant Anti-Flag Tag antibody (Mouse mAb)
Product Information
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Protein full name |
Flag Tag |
|
Synonyms |
DDDDK tag, DYKDDDDK, ddk, DDDDK epitope tag, ECS epitope tag, Enterokinase Cleavage Site tag, FLAG tag, FLAG |
|
Immunogen |
KLH conjugated Synthetic peptide corresponding to DYKDDDDK |
|
Isotype |
IgG |
|
Purity |
Affinity purification |
Applications
|
WB |
Species independent |
1: 500-1: 2000 |
Flag-Tag fusion protein |
|
ICC/IF |
Species independent |
1: 500-1: 1000 |
Transfected HEK-293T cells |
|
IP |
Species independent |
1: 50-1: 200 |
transfected HEK-293F cells |
Special note
Epitope labeling has been proved to be an effective method that can be performed on target proteins. Tag antibody can be used in a variety of applications, including ChIP, CoIP, ELISA, FC, IF, IHC, IP, WB and so on.
Background
The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion.The DDDDK peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the DDDDK peptide is more likely to be accessible to antibodies. A DDDDK-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on.
Images
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Western blot analysis of FLAG Tag (GB15938) at dilution of 1: 2000 |
|
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Immunocytochemistry analysis of 4% paraformaldehyde fixed transfected HEK-293T cells using FLAG Tag (GB15938) at dilution of 1: 600 |
|
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Immunoprecipitation of transfected HEK-293F cells lysate using Flag-Tag（IP: GB15938, 1: 100; Detection: GB11938, 1: 3000） |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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