Anti-Occludin Rabbit pAb
Product Information
|
Description |
Occludin rabbit polyclonal antibody |
|
Protein full name |
Occludin |
|
Synonyms |
OCLN, Tight junction protein occludin, PTORCH1, PPP1R115, Phosphatase 1 regulatory subunit 115, Ocln |
|
Immunogen |
KLH conjugated Synthetic peptide corresponding to Human Occludin |
|
Isotype |
IgG |
|
Purity |
Affinity purification |
|
Subcellular location |
Cell membrane |
|
Predicted MW. |
59 kDa |
|
Observed MW. |
59 kDa |
|
Uniprot ID |
Q16625, Q61146, Q6P6T5 |
Applications
|
WB |
Mouse, Rat |
1: 500-1: 1000 |
colon, brain, kidney |
|
IHC/IF |
Human, Mouse, Rat |
1: 500-1: 1500 |
appendicitis, colon, colon cancer |
Background
Occludin is a 65-kDa (522-amino acid polypeptide -human) integral plasma-membrane protein located at the tight junctions, described for the first time in 1993 by Shoichiro Tsukita.Together with the Claudin group of proteins, it is the main component of the tight junctions.
Images
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WB analysis of Occludin (GB111401). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 3000, RT, 1h. |
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IHC analysis of Occludin-1 (GB111401). Sample: Human colon cancer (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 750, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
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IHC analysis of occludin1 (GB111401). Sample: Rat colon (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 750, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
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IF analysis of occludin (GB111401). Sample: Human appendicitis (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH 9.0) (G1203),98℃,20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 750, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h. |
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IF analysis of occludin (GB111401). Sample: Mouse small intestine (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH 9.0) (G1203),98℃,20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 750, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h. |
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|
IF analysis of occludin (GB111401). Sample: Rat colon (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH 9.0) (G1203),98℃,20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 750, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h. |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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