Product Information
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Protein full name |
Proliferation marker protein Ki-67 |
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Synonyms |
MKI67, marker of proliferation Ki-67, KIA,MIB-1, PPP1R105 |
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Uniprot ID |
P46013 |
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Immunogen |
Human recombinant Ki67 protein expressed in HEK293 cells |
|
Isotype |
IgG1, κ |
|
Clone Number |
SB231 |
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Predicted MW./Observed MW. |
359 kDa / 359 kDa |
|
Purity |
Affinity purification |
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Subcellular location |
Nucleus |
Applications
| Applications | Species | Dilution | Positive Tissue |
| WB | Human, Mouse, Rat | 1: 300-1: 500 | NIH/3T3 |
| IHC/IF | Human | 1: 1000-1: 3000 | colon cancer, lung cancer, lymph node |
Background
Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly . Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface . Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility .
Images
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WB analysis of Ki67(GB150023). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 300, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 5000, RT, 1h. |
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IHC analysis of Ki67(GB150023). Sample: Human colon cancer, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 2000, 4℃ overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG (H+L), Ready to use(G1301), RT, 20min. |
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IHC analysis of Ki67(GB150023). Sample: Human esophageal carcinoma, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 2000, 4℃ overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG (H+L), Ready to use(G1301), RT, 20min. |
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IHC analysis of Ki67(GB150023). Sample: Human gastric carcinoma, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 2000, 4℃ overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG (H+L), Ready to use(G1301), RT, 20min. |
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IHC analysis of Ki67(GB150023). Sample: Human lymph node, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 2000, 4℃ overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG (H+L), Ready to use(G1301), RT, 20min. |
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IHC analysis of Ki67(GB150023). Sample: Human stomach, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 2000, 4℃ overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG (H+L), Ready to use(G1301), RT, 20min. |
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IF analysis of Ki67(GB150023)red. Sample: Human colon cancer, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG (H+L) (GB21301), 1: 300, RT, 1h. |
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IF analysis of Ki67(GB150023)red. Sample: Human lung cancer, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG (H+L) (GB21301), 1: 300, RT, 1h. |
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IF analysis of Ki67(GB150023)red. Sample: Human tonsil, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG (H+L) (GB21301), 1: 300, RT, 1h. |
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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