Anti-CD31 Mouse mAb
Product Information
|
Protein full name |
Platelet endothelial cell adhesion molecule |
|
Synonyms |
CD31 Antigen, EndoCAM, PECAM-1, GPIIA, PECA1, CD31 |
|
Immunogen |
KLH conjugated Synthetic peptide corresponding to Mouse CD31 |
|
Uniprot ID |
Q08481 |
|
Isotype |
IgG2a,κ |
|
Purity |
Affinity purification |
|
Subcellular location |
Membrane, Cell junction |
Applications
|
IHC/IF |
Mouse, Rat |
1: 200-1: 1000 |
lung, heart, skeletal muscle |
Background
Platelet endothelial cell adhesion molecule (PECAM-1) also known as cluster of differentiation 31 (CD31) is a protein that in humans is encoded by the PECAM1 gene found on chromosome 17. PECAM-1 plays a key role in removing aged neutrophils from the body. PECAM-1 is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte transmigration, angiogenesis, and integrin activation.
Images
|
|
IHC analysis of CD31 (GB12063). Sample: Rat skeletal muscle (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 200, 4℃ overnight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT 1h. |
|
|
IF analysis of CD31 (GB12063). Sample: Mouse lung (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 600, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-mouse IgG (H+L)(GB21301),1: 200 RT 1h. |
|
|
IF analysis of CD31 (GB12063). Sample: Mouse heart (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 600, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-mouse IgG (H+L)(GB21301),1: 200 RT 1h. |
 |
IF analysis of CD31 (GB12063). Sample: Mouse lung (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 600, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-mouse IgG (H+L)(GB21301),1: 200 RT 1h. |
 |
IF analysis of CD31 (GB12063). Sample: Rat lung (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 600, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-mouse IgG (H+L)(GB21301),1: 200 RT 1h. |
|
|
IF analysis of CD31 (GB12063). Sample: Rat heart (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 600, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-mouse IgG (H+L)(GB21301),1: 200 RT 1h. |
 |
IF analysis of CD31 (GB12063). Sample: Rat lung (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 600, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-mouse IgG (H+L)(GB21301),1: 200 RT 1h. |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
Use collapsible tabs for more detailed information that will help customers make a purchasing decision.
Ex: Shipping and return policies, size guides, and other common questions.
Français
English
