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Anti-CD8 alpha Rabbit pAb, Primary Antibody
AO-03-GB114196-50
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Product Information
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Protein full name |
T-cell surface glycoprotein CD8 alpha chain |
|
Synonyms |
CD8A, CD8, Leu2, MAL, p32,CD8a molecule, CD8 alpha |
|
Immunogen |
Recombinant protein corresponding to mouse CD8a |
|
Uniprot ID |
P01731 |
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Isotype |
IgG |
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Purity |
Affinity purification |
|
Subcellular location |
Cell membrane |
|
Predicted MW. / Observed MW. |
29 kDa / 35-37 kDa |
Applications
|
WB |
Mouse |
1: 500- 1: 1000 |
spleen, lymph node, thymus |
|
IHC/IF |
Mouse |
1: 500- 1: 1000 |
liver cancer, lymph node |
Background
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain.
Images
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WB analysis of CD8a (GB114196). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 3000, RT, 1h. |
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IHC analysis of cd8 (GB114196). Sample: Mouse liver cancer (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH 9.0) (G1203),98℃,20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
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IHC analysis of cd8 (GB114196). Sample: Mouse lymph node (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH 9.0) (G1203),98℃,20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
: AO-03-GB114196-50
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