Product Information
|
Protein full name |
High mobility group protein B1 |
|
Synonyms |
HMGB1, HMG1, HMG3, SBP-1, HMG-1, high mobility group box 1 |
|
Uniprot ID |
P63158 |
|
Immunogen |
KLH conjugated Synthetic peptide corresponding to Mouse HMGB1 |
|
Predicted MW./Observed MW. |
25 kDa / 25 kDa |
|
Purity |
Affinity purification |
|
conjugation |
HRP |
Applications
| Applications | Species | Dilution | Positive Tissue |
| WB | Human, Mouse, Rat | 1: 500-1: 1000 | NIH3T3, kidney, PC-12 |
| Incubation condition | After blocking, incubate the membrane with HRP conjugated Anti-HMGB1 antibody(ZB11103-HRP) for 3-4 hours at room temperature (20-25℃) or overnight at 4°C, and then wash the membrane 3 times for 5 mins each with TBST. | ||
Background
Like the histones, HMGB1 is among the most important chromatin proteins. In the nucleus HMGB1 interacts withnucleosomes, transcription factors, and histones. This nuclear protein organizes the DNA and regulates transcription.After binding, HMGB1 bendsDNA, which facilitates the binding of other proteins. HMGB1 supports transcription of many genes in interactions with many transcription factors. It also interacts with nucleosomes to loosen packed DNA and remodel the chromatin. Contact with core histones changes the structure of nucleosomes.
Images
|
|
WB analysis of HMGB1(ZB11103-HRP). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Antibody: HRP conjugated anti-HMGB1 antibody 1: 500, 4℃ overnight. |
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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