Product Information
Protein full name | Catenin beta-1 |
Synonyms | Beta catenin, beta-Catenin, Catenin beta 1, CTNNB, CTNNB1, PRO2286, β catenin, β-catenin, Cadherin associated protein, armadillo, catenin beta 1, CATNB |
Uniprot ID | P35222 |
Immunogen | Recombinant Human beta catenin protein expressed in E.coil |
Predicted MW./Observed MW. | 85 kDa / 92 kDa |
Purity | Affinity purification |
conjugation | HRP |
Applications
| Applications | Species | Dilution | Positive Tissue |
| WB | Human, Mouse, Rat | 1: 500-1: 1000 | A-431, brain |
| Incubation condition | After blocking, incubate the membrane with HRP conjugated Anti-beta Catenin antibody(ZB150016-HRP) for 3-4 hours at room temperature (20-25℃) or overnight at 4°C, and then wash the membrane 3 times for 5 mins each with TBST. | ||
Background
β-catenin is a dual function protein, involved in regulation and coordination of cell–cell adhesion and gene transcription. In humans, the CTNNB1 protein is encoded by the CTNNB1 gene. In Drosophila, the homologous protein is called armadillo. β-catenin is a subunit of the cadherin protein complex and acts as an intracellular signal transducer in the Wnt signaling pathway. It is a member of the catenin protein family and homologous to γ-catenin, also known as plakoglobin.
Images
| WB analysis of beta Catenin(ZB150016-HRP). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Antibody: HRP conjugated anti-beta Catenin antibody 1: 500, 4℃ overnight. |
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. The color of the product is light yellow. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).
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