Anti-Vimentin antibody (HRP conjugated)

Anti-Vimentin antibody (HRP conjugated)

Antibodies-Oncology
AO-03-ZB11192-HRP-50
VIM,CTRCT30,HEL113,vimentin
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Product Information


Protein full name

Vimentin

Synonyms

VIM, CTRCT30, HEL113, vimentin

Uniprot ID

P20152

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse vimentin

Predicted MW./Observed MW.

54 kDa / 57 kDa

Purity

Affinity purification

conjugation

HRP



Applications


Applications Species Dilution Positive Tissue
WB Human, Mouse, Rat 1: 500-1: 1000 lung, PC-12, Hep G2
Incubation condition After blocking, incubate the membrane with HRP conjugated anti-Vimentin antibody(ZB11192-HRP) for 3-4 hours at room temperature (20-25℃) or overnight at 4°C, and then wash the membrane 3 times for 5 mins each with TBST



Background

Vimentin is a type III intermediate filament (IF) protein that is expressed in mesenchymal cells. IF proteins are found in all metazoan cells as well as bacteria. IF, along with tubulin-based microtubules and actin-based microfilaments, comprises the cytoskeleton. All IF proteins are expressed in a highly developmentally-regulated fashion; vimentin is the major cytoskeletal component of mesenchymal cells. Because of this, vimentin is often used as a marker of mesenchymally-derived cells or cells undergoing an epithelial-to-mesenchymal transition (EMT) during both normal development and metastatic progression.



Images

01HlR9vRNIPvd4L.jpg

 WB analysis of Vimentin(ZB11192-HRP).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Antibody: HRP conjugated anti-Vimentin antibody 1: 500, 4℃ overnight.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


Antibodies-Oncology
: AO-03-ZB11192-HRP-50
: 10 Produits
Hurry! only 10 items left in stock.

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