Product Information
Protein full name | Voltage-dependent anion-selective channel protein 1 |
Synonyms | VDAC1, PORIN, VDAC-1, VDAC,voltage dependent anion channel 1 |
Uniprot ID | Q60932 |
Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse VDAC1 |
Predicted MW./Observed MW. | 31 kDa / 31 kDa |
Purity | Affinity purification |
conjugation | HRP |
Applications
| Applications | Species | Dilution | Positive Tissue |
| WB | Human, Mouse, Rat | 1: 500-1: 1000 | HeLa, A-431, liver |
| Incubation condition | After blocking, incubate the membrane with HRP conjugated Anti-VDAC1 / Porin antibody (ZB111939-HRP) for 3-4 hours at room temperature (20-25℃) or overnight at 4°C, and then wash the membrane 3 times for 5 mins each with TBST. | ||
Background
VDAC1, also named as VDAC, porin 31HM, porin 31HL and plasmalemmal porin, belongs to the eukaryotic mitochondrial porin family. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV, to form a channel through the mitochondrial outer membrane and also the plasma membrane. Unlike other membrane transport proteins, porins are large enough to allow passive diffusion. Studies have shown that VDAC1 is subject to both phosphorylation and acetylation.
Images
| WB analysis of VDAC1/Porin(ZB111939-HRP). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Antibody: HRP conjugated anti-VDAC1/Porin antibody 1: 500, 4℃ overnight. |
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. The color of the product is light yellow. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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