Recombinant Anti-alpha Tubulin antibody (HRP Conjugated)

Recombinant Anti-alpha Tubulin antibody (HRP Conjugated)

Antibodies-Oncology
AO-03-ZB15200-HRP-50
TUBA1A,B-ALPHA-1,LIS3,TUBA3,tubulin alpha 1a,TUBA1B,K-ALPHA-1,tubulin alpha 1b,Tublin,α-tubulin,Tubulin alpha-1A chain
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Product Information


Protein full name

Tubulin alpha-1A chain

Synonyms

TUBA1A, B-ALPHA-1, LIS3, TUBA3, tubulin alpha 1a,TUBA1B, K-ALPHA-1, tubulin alpha 1b

Uniprot ID

P68369

Immunogen

Recombinant protein corresponding to Mouse alpha Tubulin

Predicted MW./Observed MW.

50 kDa / 55 kDa

Purity

Affinity purification

conjugation

HRP



Applications


Applications Species Dilution Positive Tissue
WB Human, Mouse, Rat 1: 3000-1: 6000 HeLa, Hep G2, lung
Incubation condition After blocking, incubate the membrane with HRP conjugated Anti-alpha Tubulin antibody(ZB15200-HRP) for 3-4 hours at room temperature (20-25℃) or overnight at 4°C, and then wash the membrane 3 times for 5 mins each with TBST.



Background

Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species.



Images

01sPvEuJNwn8r5T.jpg

 WB analysis of alpha Tubulin(ZB15200-HRP).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Antibody: HRP conjugated anti-alpha Tubulin antibody 1: 3000, 4℃ overnight.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


Antibodies-Oncology
: AO-03-ZB15200-HRP-50
: 10 Produits
Hurry! only 10 items left in stock.

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