Recombinant Anti-COX IV antibody (HRP Conjugated)

Recombinant Anti-COX IV antibody (HRP Conjugated)

Antibodies-Oncology
AO-03-ZB15250-HRP-50
Cytochrome c oxidase polypeptide IV,Cytochrome c oxidase subunit IV isoform 1,COX IV-1,Cox4i1,Cox4, Cox4a,COX4I1,COX4,COX4-1,COXIV,COXIV-1,COXIV-1,cytochrome c oxidase subunit 4I1,Cytochrome c oxidase subunit 4 isoform 1,mitochondrial,COX Ⅳ,cox9
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Ajouter souhaits

Product Information


Protein full name

Cytochrome c oxidase subunit 4 isoform 1, mitochondrial

Synonyms

Cytochrome c oxidase polypeptide IV, Cytochrome c oxidase subunit IV isoform 1, COX IV-1, Cox4i1, Cox4, Cox4a, COX4I1, COX4, COX4-1, COXIV, COXIV-1, COXIV-1, cytochrome c oxidase subunit 4I1

Uniprot ID

P19783

Immunogen

Recombinant protein corresponding to Mouse COX IV

Predicted MW./Observed MW.

20 kDa / 17 kDa

Purity

Affinity purification

conjugation

HRP



Applications


Applications Species Dilution Positive Tissue
WB Mouse, Rat 1: 1000-1: 2000 brain, liver
Incubation condition After blocking, incubate the membrane with HRP conjugated Anti-COX IV antibody(ZB15250-HRP) for 3-4 hours at room temperature (20-25℃) or overnight at 4°C, and then wash the membrane 3 times for 5 mins each with TBST.



Background

COX4I1, also named as COX4 and COXIV-1, belongs to the cytochrome c oxidase IV family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COX4I1 is a marker for mitochondria. It has two isoforms (isoform 1 and 2). Isoform 1(COX4I1) is ubiquitously expressed and isoform 2 is highly expressed in lung tissues. COX4I1 is commonly used as a loading control. This antibody was generated against full length COX4I1 protein and cross reacts with COX4I2.



Images

01yNEDMXShUW4LG.jpg

 WB analysis of COX IV(ZB15250-HRP).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Antibody: HRP conjugated anti-COX IV antibody 1: 2000, 4℃ overnight.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


Antibodies-Oncology
: AO-03-ZB15250-HRP-50
: 10 Produits
Hurry! only 10 items left in stock.

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