Product Information
Protein full name | Heat shock 70 kDa protein 1A |
Synonyms | HSP70-1, HSP70.1, HSPA1, HSP72, Heat Shock 70 KDa Protein 1 |
Uniprot ID | P0DMV8 |
Immunogen | Recombinant protein corresponding to Human HSP70 |
Predicted MW./Observed MW. | 70 kDa / 70 kDa |
Purity | Affinity purification |
conjugation | HRP |
Applications
| Applications | Species | Dilution | Positive Tissue |
| WB | Human, Mouse, Rat | 1: 500-1: 2000 | brain, HeLa, Hep G2 |
| Incubation condition | After blocking, incubate the membrane with HRP conjugated Anti-HSP70 antibody(ZB15241-HRP) for 3-4 hours at room temperature (20-25℃) or overnight at 4°C, and then wash the membrane 3 times for 5 mins each with TBST. | ||
Background
This protein is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.
Images
| WB analysis of HSP70(ZB15241-HRP). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Antibody: HRP conjugated anti-HSP70 antibody 1: 2000, 4℃ overnight. |
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. The color of the product is light yellow. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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