Product Information
Protein full name | MYC Tag |
Synonyms | c-myc, Myc, MYC, Myc Epitope Tag, Myc tag, MYC tag, EQKLISEEDL, tag, Tag, TAG |
Immunogen | KLH conjugated Synthetic peptide corresponding to EQKLISEEDL |
Purity | Affinity purification |
conjugation | HRP |
Applications
| Applications | Species | Dilution | Positive Tissue |
| WB | Species independent | 1: 500-1: 1000 | The MYC-Tag fusion protein |
| Incubation condition | After blocking, incubate the membrane with HRP conjugated Anti-MYC Tag antibody(ZB15076-HRP) for 3-4 hours at room temperature (20-25℃) or overnight at 4°C, and then wash the membrane 3 times for 5 mins each with TBST. | ||
Background
The myc-tag is a short synthetic polypeptide sequence derived from c-myc protein that can be added to recombinant proteins to enable isolation and study when an antibody is not available. This antibody recognizes the MYC tag EQKLISEEDL. Using recombinant DNA technology, the myc tag can be fused to the protein and then an antibody against the myc tag can be used to probe, including purification using chromatography, tracking the protein in localization studies using immunofluorescence, or quantifying levels using Western Blot. The short polypeptide sequence can be fused to the N-terminus or the C-terminus of any protein without influencing function, although it is advised to avoid fusing it to a secretory signal.
Images
| WB analysis of MYC Tag(ZB15076-HRP). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Antibody: HRP conjugated anti-MYC Tag antibody 1: 1000, 4℃ overnight. |
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. The color of the product is light yellow. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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