Recombinant Anti-GAPDH antibody (HRP Conjugated)

Recombinant Anti-GAPDH antibody (HRP Conjugated)

Antibodies-Oncology
AO-03-ZB15004-HRP-100
直标,G3PD,HEL-S-162eP,ZB15004HRP,直标内参,GAPD,glyceraldehyde-3-phosphate dehydrogenase,GAPDH,gadph,Glyceraldehyde-3-phosphate dehydrogenase,gpdh,gapdh抗体,gaodh
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Product Information


Protein full name

Glyceraldehyde-3-phosphate dehydrogenase

Synonyms

GAPDH, GAPD, G3PD, HEL-S-162eP, glyceraldehyde-3-phosphate dehydrogenase

Uniprot ID

P16858

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse GAPDH

Predicted MW./Observed MW.

36 kDa / 36 kDa

Purity

Affinity purification

conjugation

HRP



Applications


ApplicationsSpeciesDilutionPositive Tissue
WBHuman, Mouse, Rat, Pg, Ch, Bv, Sh, Gt, Mk, Rb, Ta, Frog1: 1000-1: 3000HeLa, HepG2, brain, etc.
Incubation conditionAfter blocking, incubate the membrane with HRP conjugated anti-GAPDH antibody (ZB15004-HRP) for 2-3 hours at room temperature(20-25℃) or overnight at 4°C, and then wash the membrane 3 times for 5 mins each with TBST.



Background


Glyceraldehyde 3-phosphate dehydrogenase (abbreviated as GAPDH or less commonly as G3PDH) is an enzyme of ~37kDa that catalyzes the sixth step of glycolysis and thus serves to break downglucose for energy and carbon molecules. In addition to this long established metabolic function, GAPDH has recently been implicated in several non-metabolic processes, including transcription activation, initiation of apoptosis, ER to Golgi vesicle shuttling, and fast axonal, or axoplasmic transport. In sperm, a testis-specific isoenzyme GAPDHS is expressed.



Images

01pMuDRX5VZPOIS.jpg                        

WB analysis of (GAPDH)ZB15004-HRP.
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Antibody: HRP conjugated anti-GAPDH antibody 1:3000, 4℃ overnight.

02ObPpyGy1SzZtm.jpg                        

WB analysis of (GAPDH)ZB15004-HRP.
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Antibody: HRP conjugated anti-GAPDH antibody 1:3000, 4℃ overnight.



Storage


Storage            Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer            PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. The color of the product is light yellow. 


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


Antibodies-Oncology
: AO-03-ZB15004-HRP-100
: 10 Produits
Hurry! only 10 items left in stock.

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