Recombinant Anti-pan Cytokeratin antibody (Mouse mAb)

Protein full name

pan Cytokeratin

Synonyms

pan ck, pan-ck

Immunogen

Callus on human skin

Uniprot ID

P13647 P13645

Isotype

IgG1, κ

Clone Number

SB745

Purity

Affinity purification

Subcellular location

Cytoplasm

Predicted MW./Observed MW.

40-67 kDa / 40-67 kDa

Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1: 500-1: 1000

skin, HeLa, A431

IHC/IF

Human, Mouse, Rat

1: 1400-1: 4200

lung cancer, colon cancer, small intestine

Storage

Storage

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Storage Buffer

PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

background

Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells.

Images

WB analysis of pan Cytokeratin(GB152053).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 5000, RT, 1h.

IHC analysis of pan Cytokeratin(GB152053).
Sample: Human colon cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Antigen retrieval instrument: (ARI-4),Tris-EDTA buffer (pH 9.0) (G1203), Water bath 100℃ 25min.
Primary antibody: 1: 1400, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG (H+L), Ready to use(G1301), RT, 20min.

IF analysis of pan Cytokeratin(GB152053)red.
Sample Rat small intestine, 4% PFA (G1101) 12-24h.
Antigen retrieval: Antigen retrieval instrument: (ARI-4),Tris-EDTA buffer (pH 9.0) (G1203), Water bath 100℃ 25min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 1400, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG (H+L) (GB21301), 1: 300, RT, 1h.