Recombinant Anti-Intestinal Alkaline phosphatase antibody (Rabbit mAb)

Recombinant Anti-Intestinal Alkaline phosphatase antibody (Rabbit mAb)

Antibodies-Oncology
AO-03-GB150241-100
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Product Information

Protein full name

Alkaline phosphatase

Synonyms

Alpi, Alkaline phosphatase, Intestinal Alkaline phosphatase, IAP

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse Alkaline phosphatase

Uniprot ID

F8VPQ6

Isotype

IgG

Clone Number

SB749

Purity

Affinity purification

Subcellular location

Cell membrane

Applications

Species

Dilution

Positive Tissue

IHC/IF

Human, Mouse, Rat

1: 50-1: 200

small intestine

Storage

Storage

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Storage Buffer

PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

background

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The intestinal alkaline phosphatase gene encodes a digestive brush-border enzyme. This enzyme is a component of the gut mucosal defense system and is thought to function in the detoxification of lipopolysaccharide, and in the prevention of bacterial translocation in the gut.

Images

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IHC analysis of Intestinal Alkaline phosphatase(GB150241).
Sample: Human small intestine, 4% PFA (G1101) 12-24h.
Antigen retrieval: Antigen retrieval instrument: (ARI-4),Tris-EDTA buffer (pH 9.0) (G1203), Water bath 100℃ 25min.
Primary antibody: 1: 100, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

IHC analysis of Intestinal Alkaline phosphatase(GB150241).
Sample: Mouse small intestine, 4% PFA (G1101) 12-24h.
Antigen retrieval: Antigen retrieval instrument: (ARI-4),Tris-EDTA buffer (pH 9.0) (G1203), Water bath 100℃ 25min.
Primary antibody: 1: 150, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

IHC analysis of Intestinal Alkaline phosphatase(GB150241).
Sample: Rat small intestine, 4% PFA (G1101) 12-24h.
Antigen retrieval: Antigen retrieval instrument: (ARI-4),Tris-EDTA buffer (pH 9.0) (G1203), Water bath 100℃ 25min.
Primary antibody: 1: 200, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

For Research Use Only!                                               Ver. No.: V2.0-20260430


Antibodies-Oncology
: AO-03-GB150241-100
: 10 Produits
Hurry! only 10 items left in stock.

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