Recombinant Anti-Galectin 3 antibody (Mouse mAb)

Protein full name

Galectin-3

Synonyms

LGALS3, CBP35, GAL3, GALBP, GALIG, L31, LGALS2, MAC2, lectin, Galactoside binding soluble 3, Galectin 3, Gal-3, 35 kDa lectin, L-31, Galactose-specific lectin 3, IgE-binding protein

Immunogen

Recombinant protein corresponding to Mouse Galectin 3

Uniprot ID

P16110

Isotype

IgG1, κ

Clone Number

SB124

Purity

Affinity purification

Subcellular location

Cytoplasm, Nucleus, Secreted

Predicted MW./Observed MW.

28 kDa / 30 kDa

Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1: 500-1: 1000

colon , RAW264.7 , MCF7

IHC/IF

Human, Mouse, Rat

1: 500-1: 2000

liver, lung

Storage

Storage

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Storage Buffer

PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

background

Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity). In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells.

Images

WB analysis of Galectin 3(GB15246).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 5000, RT, 1h.

IHC analysis of Galectin 3(GB15246).
Sample: Human tonsil, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h.

IHC analysis of Galectin 3(GB15246).
Sample: Mouse lung, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h.