Recombinant Anti-Thymidylate Synthase antibody (Mouse mAb)

Protein full name

Thymidylate synthase

Synonyms

HsT422, Thymidylate synthase, thymidylate synthetase, TMS, TS, TSase, TYMS

Immunogen

Recombinant protein corresponding to Human Thymidylate synthase

Uniprot ID

P04818

Isotype

IgG

Clone Number

SB711

Purity

Affinity purification

Predicted MW./Observed MW.

36 kDa / 36 kDa

Applications

Species

Dilution

Positive Tissue

WB

Human

1: 500-1: 1000

JurKat, HeLa, 293T

Storage

Storage

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Storage Buffer

PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

background

Catalyzes the reductive methylation of 2'-deoxyuridine 5'-monophosphate (dUMP) to thymidine 5'-monophosphate (dTMP), using the cosubstrate, 5,10- methylenetetrahydrofolate (CH2H4folate) as a 1-carbon donor and reductant and contributes to the de novo mitochondrial thymidylate biosynthesis pathway.

Images

WB analysis of Thymidylate Synthase(GB152055).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 5000, RT, 1h.