Anti-TMEM16A/DOG1 Rabbit pAb

Protein full name

Anoctamin-1

Synonyms

Ano1, ANO1, DOG1, ORAOV2, TAOS2, TMEM16A, Anoctamin 1

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse TMEM16A

Uniprot ID

Q8BHY3

Isotype

IgG

Purity

Affinity purification

Predicted MW./Observed MW.

111 kDa / 111 kDa

Applications

Species

Dilution

Positive Tissue

WB

Rat

1: 500-1: 1000

liver, muscle

Storage

Storage

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Storage Buffer

PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

background

Anoctamin-1 (ANO1) also known as Transmembrane member 16A (TMEM16A) is a protein encoded by the ANO1 gene. Anoctamin-1 is a voltage-sensitive calcium-activated chloride channel that is expressed in smooth muscle and epithelial cells; it is highly expressed in human interstitial cells of Cajal (ICC) throughout the gastrointestinal tract. Changes in ANO1 channel activity directly/positively correlate with ICC activity.

Images

WB analysis of TMEM16A/DOG1(GB11920).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.