Recombinant Anti-MUC2 antibody (Rabbit mAb)

Protein full name

Mucin-2

Synonyms

MUC2, MLP, MUC-2, SMUC, mucin 2, oligomeric mucus/gel-forming, Intestinal mucin 2, MUC 2, Mucin 2

Immunogen

Recombinant protein corresponding to Human MUC2

Uniprot ID

Q02817

Isotype

IgG

Clone Number

SB628

Purity

Affinity purification

Subcellular location

Secreted

Predicted MW./Observed MW.

550 KDa / 250-350 kDa

Applications

Species

Dilution

Positive Tissue

WB

Mouse, Rat

1: 250-1: 500

colon

IHC/IF

Human, Mouse, Rat

1: 2000-1: 4000

colon, small intestine

Storage

Storage

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Storage Buffer

PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

background

This gene encodes a member of the mucin protein family. The protein encoded by this gene, also called mucin 2, is secreted onto mucosal surfaces.Mucin 2 is particularly prominent in the gut where it is secreted from goblet cells in the epithelial lininginto the lumen of the large intestine. There, mucin 2, along with small amounts of related-mucin proteins,polymerizes into a gel of which 80% by weight is oligosaccharide side-chains that are added as post-translational modifications to the mucin proteins.

Images

WB analysis of MUC2(GB150174).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 250, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

IHC analysis of MUC2(GB150174).
Sample: Human colon, 4% PFA (G1101) 12-24h.
Antigen retrieval: Antigen retrieval instrument: (ARI-4), Tris-EDTA buffer (pH 9.0) (G1203), Water bath 100℃ 25min.
Primary antibody: 1: 2000, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

IF analysis of MUC2(GB150174)green .
Sample: Rat small intestine, 4% PFA (G1101) 12-24h.
Antigen retrieval: Antigen retrieval instrument: (ARI-4), Tris-EDTA buffer (pH 9.0) (G1203), Water bath 100℃ 25min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 2000, 4℃ overnight.
Secondary antibody: 488 conjugated Goat Anti-Rabbit IgG (H+L) (GB25303), 1: 400, RT, 1h.