Recombinant Anti-MART-1 antibody (Mouse mAb)

Protein full name

Melanoma antigen recognized by T-cells 1

Synonyms

MART-1, Antigen LB39-AA, Antigen SK29-AA, Protein Melan-A, MLANA, MART1, Melanoma antigen recognized by T-cells 1

Immunogen

Recombinant protein corresponding to Human MART-1

Uniprot ID

Q16655

Isotype

IgG1, κ

Clone Number

SB576

Purity

Affinity purification

Subcellular location

Melanosome, Cytoplasm

Applications

Species

Dilution

Positive Tissue

IHC/IF

Human

1: 500-1: 1000

melanoma

Storage

Storage

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Storage Buffer

PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

background

Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes.

Images

IHC analysis of MART-1(GB150151).
Sample: Human melanoma, 4% PFA (G1101) 12-24h.
Antigen retrieval: Antigen retrieval instrument: (ARI-4),Tris-EDTA buffer (pH 9.0) (G1203), Water bath 100℃ 25min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG (H+L), Ready to use(G1301), RT, 20min.

IHC analysis of MART-1(GB150151).
Sample: Human melanoma, 4% PFA (G1101) 12-24h.
Antigen retrieval: Antigen retrieval instrument: (ARI-4),Tris-EDTA buffer (pH 9.0) (G1203), Water bath 100℃ 25min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG (H+L), Ready to use(G1301), RT, 20min.

IHC analysis of MART-1(GB150151).
Sample: Human melanoma, 4% PFA (G1101) 12-24h.
Antigen retrieval: Antigen retrieval instrument: (ARI-4),Tris-EDTA buffer (pH 9.0) (G1203), Water bath 100℃ 25min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG (H+L), Ready to use(G1301), RT, 20min.