Recombinant Anti-Melanoma gp100 antibody (Mouse mAb)

Protein full name

Melanocyte protein PMEL

Synonyms

95 kDa melanocyte specific secreted glycoprotein, 95 kDa melanocyte-specific secreted glycoprotein, D12S53E, gp100, M-beta, ME20, ME20 M/ME20 S, ME20-M, ME20-S, ME20M, ME20M/ME20S, ME20S, Melanocyte lineage specific antigen GP100, Melanocyte protein mel 17, Melanocyte protein Pmel 17, Melanocyte protein Pmel 17 precursor, Melanocytes lineage-specific antigen GP100, Melanoma associated ME20 antigen, Melanoma gp100, Melanoma-associated ME20 antigen, Melanosomal matrix protein 17, Melanosomal matrix protein17, P1, p100, p26, PMEL 17, PMEL, Premelanosome protein, Secreted melanoma-associated ME20 antigen, SI, SIL, D12S53E, PMEL17, SILV, PMEL

Immunogen

Recombinant protein corresponding to Human Melanoma gp100

Uniprot ID

P40967

Isotype

IgG1

Clone Number

SB580

Purity

Affinity purification

Subcellular location

Melanosome, Cytoplasm

Applications

Species

Dilution

Positive Tissue

IHC/IF

Human

1: 500-1: 1500

melanoma

Storage

Storage

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Storage Buffer

PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

background

Forms physiological amyloids that play a central role in melanosome morphogenesis and pigmentation. The maturation of unpigmented premelanosomes from stage I to II is marked by assembly of processed amyloidogenic fragments into parallel fibrillar sheets, which elongate the vesicle into a striated ellipsoidal shape. In pigmented stage III and IV melanosomes, the amyloid matrix serves as a platform where eumelanin precursors accumulate at high local concentrations for pigment formation. May prevent pigmentation-associated toxicity by sequestering toxic reaction intermediates of eumelanin biosynthesis pathway.

Images

IHC analysis of Melanoma gp100(GB150160).
Sample: Human melanoma, 4% PFA (G1101) 12-24h.
Antigen retrieval: Antigen retrieval instrument: (ARI-4),Tris-EDTA buffer (pH 9.0) (G1203), Water bath 100℃ 25min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG (H+L), Ready to use(G1301), RT, 20min.

IHC analysis of Melanoma gp100(GB150160).
Sample: Human melanoma, 4% PFA (G1101) 12-24h.
Antigen retrieval: Antigen retrieval instrument: (ARI-4),Tris-EDTA buffer (pH 9.0) (G1203), Water bath 100℃ 25min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG (H+L), Ready to use(G1301), RT, 20min.

IHC analysis of Melanoma gp100(GB150160).
Sample: Human melanoma, 4% PFA (G1101) 12-24h.
Antigen retrieval: Antigen retrieval instrument: (ARI-4),Tris-EDTA buffer (pH 9.0) (G1203), Water bath 100℃ 25min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG (H+L), Ready to use(G1301), RT, 20min.