Recombinant Anti-PD-L1 antibody (Rabbit mAb)

Protein full name

Programmed cell death 1 ligand 1

Synonyms

CD274, B7-H, B7H1, PDL-1, PDCD1L1, PDCD1LG1, PDL1, CD274 molecule, Programmed cell death 1 ligand 1

Immunogen

Recombinant protein corresponding to Mouse PD-L1

Uniprot ID

Q9EP73

Isotype

IgG, κ

Clone Number

SB374

Purity

Affinity purification

Subcellular location

Cell membrane

Predicted MW. / Observed MW.

33 kDa / 40-55 kDa

Applications

Species

Dilution

Positive Tissue

WB

Mouse

1: 500-1: 1500

RAW264.7

IHC/IF

Mouse

1: 500-1: 1500

spleen, thymus gland, subcutaneous nude-mouse model of 4T1 cell

Storage

Storage

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Storage Buffer

PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

background

Plays a critical role in induction and maintenance of immune tolerance to self.As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response.Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10).Can also act as a transcription coactivator: in response to hypoxia, translocates into the nucleus via its interaction with phosphorylated STAT3 and promotes transcription of GSDMC, leading to pyroptosis.The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival.The interaction with PDCD1/PD-1 inhibits cytotoxic T lymphocytes (CTLs) effector function.

Images

WB analysis of PD-L1(GB150059).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

IHC analysis of PD-L1(GB150059).
Sample: Mouse spleen, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 100℃ 25min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

IF analysis of PD-L1(GB150059)red.
Sample: Mouse thymus gland, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 100℃ 25min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 1500, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.