Recombinant Anti-CD79A antibody (Mouse mAb)

Protein full name

B-cell antigen receptor complex-associated protein alpha chain

Synonyms

IGA, MB1, CD79A, CD79a, CD79a molecule, immunoglobulin-associated alpha

Immunogen

Recombinant protein corresponding to Human CD79A

Uniprot ID

P11912

Isotype

IgG1, κ

Clone Number

SB498

Purity

Affinity purification

Subcellular location

Cytoplasm

Predicted MW. / Observed MW.

25 kDa / 40-45 kDa

Applications

Species

Dilution

Positive Tissue

WB

Human

1: 1000-1: 2000

Raji

IHC/IF

Human

1: 1000-1: 2500

tonsil, colon

Storage

Storage

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Storage Buffer

PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

background

Required in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells. Stimulates SYK autophosphorylation and activation. Binds to BLNK, bringing BLNK into proximity with SYK and allowing SYK to phosphorylate BLNK. Also interacts with and increases activity of some Src-family tyrosine kinases. Represses BCR signaling during development of immature B-cells.

Images

WB analysis of CD79A(GB150123).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 5000, RT, 1h.

IHC analysis of CD79A(GB150123).
Sample: Human colon, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 100℃ 25min.
Primary antibody: 1: 2000, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG (H+L), Ready to use(G1301), RT, 20min.

IF analysis of CD79A(GB150123)red.
Sample: Human tonsil, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 100℃ 25min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 2000, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG (H+L) (GB21301), 1: 300, RT, 1h.