Recombinant Anti-CA19-9 antibody (Mouse mAb)

Immunogen

Recombinant protein corresponding to Human CA19-9

Isotype

IgM

Clone Number

SB503

Purity

Affinity purification

Subcellular location

Cytoplasm

Applications

Species

Dilution

Positive Tissue

IHC/IF

Human

1: 300-1: 600

Hepatobiliary cancer, colon cancer, gastric carcinoma

Storage

Storage

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Storage Buffer

PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

background

CA19-9 (Carbohydrate Antigen 19-9) is a carbohydrate antigen, belonging to the category of oligosaccharide tumor-associated antigens, and is a glycolipid on the cell membrane. It is formed from the precursor substances of Lewis antigens (including Le-a and Le-b) under the joint action of sialyltransferase and fucosyltransferase. CA19-9 is an important tumor marker, especially in the diagnosis and monitoring of pancreatic cancer, where the levels of CA19-9 are elevated in over 80% of patients with pancreatic cancer. CA19-9 can also be used in the clinical auxiliary detection of gastric cancer, colorectal cancer, gallbladder cancer, cholangiocarcinoma, and liver cancer.

Images

IHC analysis of CA19-9(GB150125).
Sample: Human Hepatobiliary Cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 100℃ 25min.
Primary antibody: 1: 300, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG (H+L), Ready to use(G1301), RT, 20min.

IHC analysis of CA19-9(GB150125).
Sample: Human colon cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 100℃ 25min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG (H+L), Ready to use(G1301), RT, 20min.

IHC analysis of CA19-9(GB150125).
Sample: Human gastric carcinoma, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 100℃ 25min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG (H+L), Ready to use(G1301), RT, 20min.