Recombinant Anti-surfactant-A antibody (Rabbit mAb)

Protein full name

Pulmonary surfactant-associated protein A1,
Pulmonary surfactant-associated protein A2

Synonyms

COLEC4, PSAP, SFTP1, SFTPA, SFTPA1B,COLEC5, PSAP, SFTP1, SFTPA, SFTPA2B

Immunogen

Recombinant protein corresponding to Human surfactant-A

Uniprot ID

Q8IWL2/Q8IWL1

Isotype

IgG

Clone Number

SB509

Purity

Affinity purification

Subcellular location

Secreted, Cytoplasm, Cell membrane

Applications

Species

Dilution

Positive Tissue

IHC/IF

Human

1: 1000-1: 2000

lung, lung cancer

Storage

Storage

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Storage Buffer

PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

background

In presence of calcium ions, it binds to surfactant phospholipids and contributes to lower the surface tension at the air-liquid interface in the alveoli of the mammalian lung and is essential for normal respiration.Enhances the expression of MYO18A/SP-R210 on alveolar macrophages.

Images

IHC analysis of surfactant-A(GB15989).
Sample: Human lung cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 100℃ 25min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

IHC analysis of surfactant-A(GB15989).
Sample: Human lung, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 100℃ 25min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

IF analysis of surfactant-A(GB15989)red.
Sample: Human lung cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 100℃ 25min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.