Recombinant Anti-MUC2 antibody (Rabbit mAb)

Recombinant Anti-MUC2 antibody (Rabbit mAb)

Antibodies-Oncology
AO-03-GB15344-50
MUC2,MLP,MUC-2,SMUC,mucin 2,oligomeric mucus/gel-forming,Intestinal mucin 2,MUC 2,Mucin 2
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Product Information


Protein full name

Mucin-2

Synonyms

MUC2, MLP, MUC-2, SMUC, mucin 2, oligomeric mucus/gel-forming, Intestinal mucin 2, MUC 2, Mucin 2

Uniprot ID

Q02817

Immunogen

KLH conjugated Synthetic peptide corresponding to Human MUC2

Isotype

IgG, κ

Clone Number

SB343

Purity

Affinity purification

Subcellular location

Secreted



Applications


Applications Species Dilution Positive Tissue
IHC/IF Human, Mouse, Rat 1: 500-1: 1500 small intestine, colon, colon cancer



Background

This gene encodes a member of the mucin protein family. The protein encoded by this gene, also called mucin 2, is secreted onto mucosal surfaces.Mucin 2 is particularly prominent in the gut where it is secreted from goblet cells in the epithelial lininginto the lumen of the large intestine. There, mucin 2, along with small amounts of related-mucin proteins,polymerizes into a gel of which 80% by weight is oligosaccharide side-chains that are added as post-translational modifications to the mucin proteins.



Images

01P6MNMIdjecYZn.jpg

 IHC analysis of MUC2(GB15344).
Sample: Human colon cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

024RwaVXPYBADti.jpg

 IHC analysis of MUC2(GB15344).
Sample: Human small intestine, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

03cNvXGAUR2psHq.jpg

 IHC analysis of MUC2(GB15344).
Sample: Mouse small intestine, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

04qkqR2ALuGGLSJ.jpg

 IHC analysis of MUC2(GB15344).
Sample: Rat small intestine, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

05nA2XzM2RrEkFK.jpg

 IF analysis of MUC2(GB15344)red.
Sample: Human small intestine, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

06KC2msTbVSVtms.jpg

 IF analysis of MUC2(GB15344)red.
Sample: Human colon cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

07YhtHSAt7l7xpe.jpg

 IF analysis of MUC2(GB15344)red.
Sample: Mouse small intestine, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

08rBwaaMD7JOWYi.jpg

 IF analysis of MUC2(GB15344)red.
Sample: Rat colon, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

092SPDYp1TLGSnM.jpg

 IF analysis of MUC2(GB15344)red.
Sample: Rat small intestine, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


Antibodies-Oncology
: AO-03-GB15344-50
: 10 Produits
Hurry! only 10 items left in stock.

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