Recombinant Anti-MUC6 antibody (Rabbit mAb)

Recombinant Anti-MUC6 antibody (Rabbit mAb)

Antibodies-Oncology
AO-03-GB150033-100
MUC-6,Mucin 6,Gastric mucin-6
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Product Information


Protein full name

Mucin-6

Synonyms

MUC-6, Mucin 6, Gastric mucin-6

Uniprot ID

Q6W4X9

Immunogen

Recombinant protein corresponding to Human Mucin-6

Isotype

IgG, κ

Clone Number

SB279

Purity

Affinity purification

Subcellular location

Secreted



Applications


Applications Species Dilution Positive Tissue
IHC/IF Human 1: 500-1: 1000 stomach



Background

May provide a mechanism for modulation of the composition of the protective mucus layer related to acid secretion or the presence of bacteria and noxious agents in the lumen. Plays an important role in the cytoprotection of epithelial surfaces and are used as tumor markers in a variety of cancers. May play a role in epithelial organogenesis.



Images

01u5ENkJoWeyJmN.jpg

 IHC analysis of MUC6(GB150033).
Sample: Human stomach, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 800, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

02h6WPvLE2QCKPK.jpg

 IHC analysis of MUC6(GB150033).
Sample: Human stomach, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 800, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

03zNP3emYu1ysMB.jpg

 IF analysis of MUC6(GB150033)red.
Sample: Human stomach, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 800, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

04gVoE7u1o5gWUY.jpg

 IF analysis of MUC6(GB150033)red.
Sample: Human stomach, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 800, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


Antibodies-Oncology
: AO-03-GB150033-100
: 10 Produits
Hurry! only 10 items left in stock.

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