Recombinant Anti-CA125/MUC16 antibody (Mouse mAb)

Recombinant Anti-CA125/MUC16 antibody (Mouse mAb)

Antibodies-Oncology
AO-03-GB15017-100
CA-125,MUC-16,MUC16,CA125,Mucin 16,Mucin-16
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Product Information


Protein full name

Mucin-16

Synonyms

CA-125, MUC-16, MUC16,CA125, Mucin 16

Uniprot ID

Q8WXI7

Immunogen

Human recombinant CA125 protein expressed in HEK293 cells

Isotype

IgG1, κ

Clone Number

SB204

Purity

Affinity purification

Subcellular location

Secreted, Cell membrane



Applications


Applications Species Dilution Positive Tissue
IHC/IF Human 1: 250-1: 1000 ovarian cancer



Background

Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.



Images

01277tYqgx9azf5.jpg

 IHC analysis of CA125/MUC16(GB15017).
Sample: Human endometrial Cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG (H+L), Ready to use(G1301), RT, 20min.

02gO5I1iZQzARcd.jpg

 IHC analysis of CA125/MUC16(GB15017).
Sample: Human endometrial Cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG (H+L), Ready to use(G1301), RT, 20min.

03UBNhGMwAAafPL.jpg

 IHC analysis of CA125/MUC16(GB15017).
Sample: Human endometrial Cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG (H+L), Ready to use(G1301), RT, 20min.

04vtceUcKKrYLFE.jpg

 IHC analysis of CA125/MUC16(GB15017).
Sample: Human lung cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG (H+L), Ready to use(G1301), RT, 20min.

05rNL7eSXhdcAP4.jpg

 IHC analysis of CA125/MUC16(GB15017).
Sample: Human lung cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG (H+L), Ready to use(G1301), RT, 20min.

06rHwyNuf0VRHFr.jpg

 IHC analysis of CA125/MUC16(GB15017).
Sample: Human ovarian cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG (H+L), Ready to use(G1301), RT, 20min.

07zA0YzOUDdC6Sy.jpg

 IHC analysis of CA125/MUC16(GB15017).
Sample: Human ovarian cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG (H+L), Ready to use(G1301), RT, 20min.

0815NdvAONyCOD4.jpg

 IHC analysis of CA125/MUC16(GB15017).
Sample: Human ovarian cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG (H+L), Ready to use(G1301), RT, 20min.

09MXSCER0CqZOkn.jpg

 IF analysis of CA125/MUC16(GB15017)red.
Sample: Human ovarian cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG (H+L) (GB21301), 1: 300, RT, 1h.

10jued41cnNOlf1.jpg

 IF analysis of CA125/MUC16(GB15017)red.
Sample: Human ovarian cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG (H+L) (GB21301), 1: 300, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


Antibodies-Oncology
: AO-03-GB15017-100
: 10 Produits
Hurry! only 10 items left in stock.

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