Anti-MMP9 Mouse mAb

Protein full name

Matrix metalloproteinase-9

Synonyms

MMP9, CLG4B, GELB, MANDP2, MMP-9, 92 kDa type IV collagenase, 92 kDa gelatinase, gelatinase B, matrix metallopeptidase 9

Immunogen

Recombinant protein corresponding to Human MMP9

Uniprot ID

P14780

Isotype

IgG2a, κ

Clone Number

2-6D8

Purity

Affinity purification

Subcellular location

Secreted

Applications

Species

Dilution

Positive Tissue

IHC/IF

Human, Monkey, Pig

1: 300-1: 600

tonsil, esophagus, lung

Storage

Storage

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Storage Buffer

PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

background

May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.

Images

IHC analysis of MMP9(GB120004).
Sample: Human tonsil, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 300, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG (H+L), Ready to use(G1301), RT, 20min.

IHC analysis of MMP9(GB120004).
Sample: Human esophagus, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 300, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG (H+L), Ready to use(G1301), RT, 20min.

IHC analysis of MMP9(GB120004).
Sample: Human lung, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 300, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG (H+L), Ready to use(G1301), RT, 20min.