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Product Information
|
Protein full name |
Alkaline phosphatase, placental type |
|
Synonyms |
Alkaline phosphatase Regan isozyme, Placental alkaline phosphatase 1, PLAP-1, ALPP, PLAP, DOA1, PLA2P, PLAA, PLAP, Glycerophosphatase |
|
Uniprot ID |
P05187 |
|
Immunogen |
Human recombinant PLAP protein expressed in HEK293 cells |
|
Isotype |
IgG1, κ |
|
Clone Number |
1G3C10 |
|
Predicted MW./Observed MW. |
58 kDa / 68 kDa |
|
Purity |
Affinity purification |
|
Subcellular location |
Cell membrane |
Applications
| Applications | Species | Dilution | Positive Tissue |
| WB | Human | 1: 500-1: 1000 | HeLa, Hep G2 |
| IHC/IF | Human | 1: 500-1: 1500 | placenta |
Background
Alkaline phosphatase that can hydrolyze various phosphate compounds.
Images
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WB analysis of Placental alkaline phosphatase (PLAP)(GB122546). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 5000, RT, 1h. |
|
|
IHC analysis of Placental alkaline phosphatase (PLAP)(GB122546). Sample: Human placenta, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h. |
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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