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Product Information
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Protein full name |
Melanoma-associated antigen E1 |
|
Synonyms |
Alpha-dystrobrevin-associated MAGE Protein, DAMAGE, Hepatocellular carcinoma-associated protein 1, MAGE-E1 antigen, MAGEE1, HCA1, KIAA1587 |
|
Uniprot ID |
Q6PCZ4 |
|
Immunogen |
Recombinant protein corresponding to Mouse MAGE 1 |
|
Isotype |
IgG |
|
Predicted MW./Observed MW. |
103 kDa / 55 kDa |
|
Purity |
Affinity purification |
Applications
| Applications | Species | Dilution | Positive Tissue |
| WB | Human, Mouse | 1: 300-1: 800 | testis, lung |
Background
May enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex.
Images
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WB analysis of MAGE 1(GB113218). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 800, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h. |
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|
WB analysis of MAGE 1(GB113218). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 800, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h. |
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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