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Product Information
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Protein full name |
Galectin-3 |
|
Synonyms |
LGALS3, CBP35, GAL3, GALBP, GALIG, L31, LGALS2, MAC2, lectin, Galactoside binding soluble 3, Galectin 3, Gal-3, 35 kDa lectin, L-31, Galactose-specific lectin 3, IgE-binding protein |
|
Uniprot ID |
P16110 |
|
Immunogen |
Recombinant protein corresponding to Mouse Galectin 3 |
|
Isotype |
IgG1, κ |
|
Clone Number |
5G11G7 |
|
Predicted MW./Observed MW. |
28 kDa / 30 kDa |
|
Purity |
Affinity purification |
|
Subcellular location |
Cytoplasm, Nucleus, Secreted |
Applications
| Applications | Species | Dilution | Positive Tissue |
| WB | Human, Rat | 1: 500-1: 1000 | colon |
| IHC/IF | Human, Mouse, Rat | 1: 2000-1: 4000 | enteritis, hepatitis, lung |
Background
Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity). In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells.
Images
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WB analysis of Galectin 3(GB140). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 5000, RT, 1h. |
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WB analysis of Galectin 3(GB140). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 5000, RT, 1h. |
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IHC analysis of Galectin 3(GB140). Sample: Human lung, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 2000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h. |
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IHC analysis of Galectin 3(GB140). Sample: Mouse pneumonia, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 2000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h. |
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IHC analysis of Galectin 3(GB140). Sample: Rat enteritis, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 2000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h. |
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|
IHC analysis of Galectin 3(GB140). Sample: Rat pneumonia, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 2000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h. |
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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