Français
English
Product Information
|
Protein full name |
Serpin B3 |
|
Synonyms |
Protein T4-A, Squamous cell carcinoma antigen 1, HsT1196, SERPINB3, SCCA, SCCA1, SCC, SCCAPD, T4A |
|
Uniprot ID |
P29508 |
|
Immunogen |
Recombinant protein corresponding to Mouse SerpinB3 |
|
Isotype |
IgG |
|
Predicted MW./Observed MW. |
45 kDa / 45 kDa |
|
Purity |
Affinity purification |
|
Subcellular location |
Cytoplasm |
Applications
| Applications | Species | Dilution | Positive Tissue |
| WB | Mouse, Rat | 1: 800-1: 1000 | liver, skin |
| IHC/IF | Human, Mouse, Rat | 1: 500-1: 2100 | esophagus, gastric cancer, uterus |
Background
Metastasis of a primary tumor to a distant site is determined through signaling cascades that break down interactions between the cell and extracellular matrix proteins. Among the proteins mediating metastasis are serine prote-ases, such as neutrophil elastase.
Images
|
|
WB analysis of SerpinB3/SCCA(GB111989). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 800, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h. |
|
|
IF analysis of SerpinB3/SCCA(GB111989)red. Sample: Human esophagus, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 650, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
|
|
IF analysis of SerpinB3/SCCA(GB111989)red. Sample: Mouse uterus, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
|
|
IF analysis of SerpinB3/SCCA(GB111989)red. Sample: Human gastric cancer, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
Use collapsible tabs for more detailed information that will help customers make a purchasing decision.
Ex: Shipping and return policies, size guides, and other common questions.
- Paste the label on a flat surface on the package
- Make sure that both 1D and 2D barcodes are clearly visible
- Ensure that the label is smooth and isn’t creased or wrinkled
- Check for any tears, dents, holes or scratches
- Pack your product tightly, with the right size packaging
- Ensure both barcodes are on a flat surface of the package