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Product Information
|
Protein full name |
CD276 antigen |
|
Synonyms |
B7 homolog 3, B7-H3, Costimulatory molecule, CD276, B7h3, 4Ig B7 H3 |
|
Uniprot ID |
Q8VE98 |
|
Immunogen |
Recombinant protein corresponding to Mouse CD276 |
|
Isotype |
IgG |
|
Predicted MW./Observed MW. |
34 kDa / 100 kDa |
|
Purity |
Affinity purification |
Applications
| Applications | Species | Dilution | Positive Tissue |
| WB | Human | 1: 500-1: 1000 | HeLa, HepG2, A431 |
Background
B7-H3 (CD276) is a type I transmembrane protein expressed on many tissues and cell types. B7-H3 is a 100-kDa glycoprotein that belongs to the B7 immunoregulatory family and participates in the regulation of T-cell-mediated immune response probably by functioning as both a T cell costimulator and coinhibitor Overexpressed on a wide range of human solid cancers, B7-H3 has been implicated in cancer progression and metastasis and becomes an attractive target for cancer immunotherapy.
Images
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WB analysis of CD276(GB111975). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h. |
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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