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Product Information
|
Protein full name |
60S ribosomal protein L5 |
|
Synonyms |
Rpl5, BA6, MSTP030, ribosomal protein L5, U21RNA, PPP1R135 |
|
Uniprot ID |
P47962 |
|
Immunogen |
Recombinant protein corresponding to Mouse RPL5 |
|
Isotype |
IgG |
|
Predicted MW./Observed MW. |
34 kDa / 34 kDa |
|
Purity |
Affinity purification |
Applications
| Applications | Species | Dilution | Positive Tissue |
| WB | Human, Mouse | 1: 500-1: 1000 | brain, lung, liver |
Background
RPL5 encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L18P family of ribosomal proteins. It is located in the cytoplasm. The protein binds 5S rRNA to form a stable complex called the 5S ribonucleoprotein particle (RNP), which is necessary for the transport of nonribosome-associated cytoplasmic 5S rRNA to the nucleolus for assembly into ribosomes. The protein interacts specifically with the beta subunit of casein kinase II.
Images
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WB analysis of RPL5(GB111928). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h. |
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|
WB analysis of RPL5(GB111928). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h. |
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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