Anti-Wilms Tumor Protein Rabbit pAb

Protein full name

Wilms tumor protein homolog

Synonyms

AEWS-GUD, NPHS4, WAGR, WIT-2, WT33, Wilms tumor, WT1

Immunogen

Recombinant protein corresponding to Mouse WT1

Uniprot ID

P22561

Isotype

IgG

Purity

Affinity purification

Subcellular location

Cytoplasm, Nucleus

Applications

Species

Dilution

Positive Tissue

IHC/IF

Human, Mouse, Rat

1: 100-1: 300

kidney, spleen, testis

Storage

Storage

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Storage Buffer

PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

background

Transcription factor that plays an important role in cellular development and cell survival. Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3' (By similarity). Regulates the expression of numerous target genes, including EPO . Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors.

Images

IHC analysis of Wilms Tumor Protein(GB11382).
Sample: Human kidney, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 200, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

IHC analysis of Wilms Tumor Protein(GB11382).
Sample: Rat kidney, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 200, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

IF analysis of Wilms Tumor Protein(GB11382)red.
Sample: Mouse kidney, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 100, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.