Recombinant Anti-Phospho-CREB(S133) antibody (Rabbit mAb)

Protein full name

Cyclic AMP-responsive element-binding protein 1

Synonyms

CREB-1, CAMP-responsive element-binding protein 1, CAMP responsive element binding protein 1, Active transcription factor CREB, P-CREB

Immunogen

KLH conjugated Synthetic phosphopeptide corresponding to Human CREB（S133）

Uniprot ID

P16220

Isotype

IgG

Clone Number

SB540

Purity

Affinity purification

Subcellular location

Nucleus

Predicted MW./Observed MW.

35 kDa / 43 kDa

Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1: 500-1: 1500

C6 cells treated with calycosin, HT-29 cells treated with calycosin

IHC/IF

Human, Mouse, Rat

1: 1000-1: 2000

heart, tonsil, kidney

Storage

Storage

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Storage Buffer

PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

background

Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells.

Images

WB analysis of Phospho-CREB(S133)(GB150137).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

IHC analysis of Phospho-CREB(S133)(GB150137).
Sample: Rat heart, 4% PFA (G1101) 12-24h.
Antigen retrieval: Antigen retrieval instrument: (ARI-4),Tris-EDTA buffer (pH 9.0) (G1203), Water bath 100℃ 25min.
Primary antibody: 1: 2000, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

IF analysis of Phospho-CREB(S133)(GB150137)red.
Sample: Human kidney, 4% PFA (G1101) 12-24h.
Antigen retrieval: Antigen retrieval instrument: (ARI-4),Tris-EDTA buffer (pH 9.0) (G1203), Water bath 90℃ 30min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 2000, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.