Recombinant Anti-Phospho-PERK (T982) antibody (Rabbit mAb)

Protein full name

Eukaryotic translation initiation factor 2-alpha kinase 3

Synonyms

EIF2AK3, PEK, PERK, WRS ,eukaryotic, p-PERK

Immunogen

KLH conjugated Synthetic phosphopeptide corresponding to Human PERK (T982)

Uniprot ID

Q9NZJ5

Isotype

IgG

Clone Number

SB519

Purity

Affinity purification

Predicted MW. / Observed MW.

125 kDa / 180 kDa

Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1: 1000-1: 2000

NIH/3T3

Storage

Storage

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Storage Buffer

PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

background

Eukaryotic translation initiation factor 2-alpha kinase 3, also known as protein kinase R (PKR)-like endoplasmic reticulum kinase (PERK), is an enzyme that in humans is encoded by the EIF2AK3 gene.The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis.

Images

WB analysis of Phospho-PERK (T982)(GB150110).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.