Anti-Phospho-AMPK alpha 1 (T183) + AMPK alpha 2 (T172) Rabbit pAb

Protein full name

5'-AMP-activated protein kinase catalytic subunit alpha-1/5'-AMP-activated protein kinase catalytic subunit alpha-2

Synonyms

AMPK, AMPK alpha 2, AMPK subunit alpha 2, AMPK2, AMPKA2, AMPKα2, PRKAA, PRKAA2, HMGCR kinase, AMPK subunit alpha-1, Acetyl-CoA carboxylase kinase, ACACA kinase, Hydroxymethylglutaryl-CoA reductase kinase, HMGCR kinase, Tau-protein kinase PRKAA1, PRKAA1, AMPK1, p-AMPK, p-AMPKα, p-AMPKα1, p-AMPKα2, p-AMPKα1/α2, p-ampk, p-ampkα, p-ampkα1, p-ampkα2, p-ampkα1/α2

Immunogen

KLH conjugated Synthetic phosphopeptide corresponding to Human AMPK alpha 1 (T183) + AMPK alpha 2 (T172)

Uniprot ID

Q13131, P54646

Isotype

IgG

Purity

Affinity purification

Predicted MW. / Observed MW.

62 kDa / 65 kDa

Applications

Species

Dilution

Positive Tissue

WB

Mouse

1: 300-1: 600

C2C12

Storage

Storage

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Storage Buffer

PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

background

The mammalian 5-prime-AMP-activated protein kinase (AMPK) appears to play a role in protecting cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. PRKAA1 is also named as AMPK1, ACACA kinase, HMGCR kinase. It is a mammalian homologue of sucrose non-fermenting protein kinase (SNF-1), which belongs to a serine/threonine protein kinase family. It has 2 isoforms with molecular mass of 63-66 kDa produced by alternative splicing.

Images

WB analysis of Phospho-AMPK alpha 1 (T183) + AMPK alpha 2 (T172)(GB114323).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 300, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.