Anti-Phospho-AMPK alpha 2(S491) Rabbit pAb

Protein full name

5'-AMP-activated protein kinase catalytic subunit alpha-2

Synonyms

AMPK, AMPK alpha 2, AMPK subunit alpha 2, AMPK2, AMPKA2, AMPKα2, PRKAA, PRKAA2, p-AMPK, p-AMPKα, p-AMPKα2, p-ampk, p-ampkα, p-ampkα2

Immunogen

KLH conjugated Synthetic phosphopeptide corresponding to Human AMPK alpha 2

Uniprot ID

P54646

Isotype

IgG

Purity

Affinity purification

Predicted MW. / Observed MW.

62 kDa / 65 kDa

Applications

Species

Dilution

Positive Tissue

WB

Human

1: 300-1: 500

HepG2, MCF7, HT-29

Storage

Storage

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Storage Buffer

PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

background

PRKAA2(protein kinase, AMP-activated, alpha 2 catalytic subunit), also named as AMPKA2, AMPK, PRKAA, AMPK2, belongs to the CAMK Ser/Thr protein kinase family and SNF1 subfamily. PRKAA2 is an αβγ heterotrimer that is activated by low cellular energy status, such as decreases in both the ATP/AMP ratio and the phosphocreatine content and it is a glycogen synthase kinase, phosphorylating Ser7 at the NH2 terminus, which decreases glycogen synthase activity .

Images

WB analysis of Phospho-AMPK alpha 2(S491)(GB114062).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 300, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

WB analysis of Phospho-AMPK alpha 2(S491)(GB114062).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 300, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.