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Product Information
| Protein full name | Serine/threonine-protein kinase mTOR |
| Synonyms | FK506-binding protein 12-rapamycin complex-associated protein 1, Mammalian target of rapamycin, Rapamycin target protein 1, FRAP, FRAP1, FRAP2, RAFT1, RAPT1, mTOR, P-MTOR |
| Uniprot ID | P42345 |
| Immunogen | KLH conjugated Synthetic phosphopeptide corresponding to Human mTOR(S2448) |
| Isotype | IgG, κ |
| Clone Number | SB424 |
| Predicted MW./Observed MW. | 289 kDa / 289 kDa |
| Purity | Affinity purification |
Applications
| Applications | Species | Dilution | Positive Tissue |
| WB | Human, Mouse, Rat | 1: 1000-1: 2000 | Hela with Calyculin A |
Background
MTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. MTOR has a calculated molecular mass of 289 kDa, and always can be detected at about 250 kDa due to some modifications.
Images
| | WB analysis of Phospho-mTOR(S2448)(GB150078). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h. |
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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