Recombinant Anti-Phospho-GSK3 Beta(S9) antibody (Rabbit mAb)
   

Recombinant Anti-Phospho-GSK3 Beta(S9) antibody (Rabbit mAb)

Antibodies-Oncology
AO-03-GB150050-50
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299,00 €
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Ajouter souhaits

Product Information


Protein full name

Glycogen synthase kinase-3 beta

Synonyms

GSK3B, Gsk3b, 7330414F15Rik, 8430431H08Rik, C86142, GSK-3, GSK-3beta, GSK3, glycogen synthase kinase 3 beta, P-GSK3β

Uniprot ID

P49841

Immunogen

Recombinant protein corresponding to Human GSK3 beta(S9)

Isotype

IgG, κ

Clone Number

SB351

Predicted MW./Observed MW.

47 kDa / 47 kDa

Purity

Affinity purification

Subcellular location

Cytoplasm, Nucleus, Cell membrane



Applications


Applications Species Dilution Positive Tissue
WB Human, Mouse, Rat 1: 1000-1: 4000 HeLa, NIH/3T3, C6
IHC/IF Human 1: 200-1: 800 lung cancer, colon cancer, renal carcinoma



Background

Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase .GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.In skeletal muscle, it contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Researches showed that the crystal structure of human GSK3B, expressed in insect cells, at 2.8-angstrom resolution .This antibody recognize the C-terminal of GSK3B.



Images

01HFwbzpQZPyFko.jpg

 WB analysis of Phospho-GSK3 Beta(S9)(GB150050).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

025kMMqhvQHdYlB.jpg

 WB analysis of Phospho-GSK3 Beta(S9)(GB150050).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

03tlSyDm4HOrfGS.jpg

 IHC analysis of Phospho-GSK3 Beta(S9)(GB150050).
Sample: Human renal carcinoma, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 200, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

04EPDnvxNfibtDq.jpg

 IHC analysis of Phospho-GSK3 Beta(S9)(GB150050).
Sample: Human colon, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 200, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

052rk2aliJgHZTv.jpg

 IHC analysis of Phospho-GSK3 Beta(S9)(GB150050).
Sample: Human colon cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 200, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

06J8pAbMbetu0Zb.jpg

 IHC analysis of Phospho-GSK3 Beta(S9)(GB150050).
Sample: Human esophageal cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 200, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

079wc3optK8XSh7.jpg

 IHC analysis of Phospho-GSK3 Beta(S9)(GB150050).
Sample: Human gastric cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 200, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

083pyct61LWQLqD.jpg

 IHC analysis of Phospho-GSK3 Beta(S9)(GB150050).
Sample: Human ovarian cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 200, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

09W4x40MnqKcqtI.jpg

 IHC analysis of Phospho-GSK3 Beta(S9)(GB150050).
Sample: Human colon cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 800, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

109L7q6m2eTdn4V.jpg

 IHC analysis of Phospho-GSK3 Beta(S9)(GB150050).
Sample: Human liver cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 800, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

11yvg9SF6ZU9ByN.jpg

 IHC analysis of Phospho-GSK3 Beta(S9)(GB150050).
Sample: Human lung cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 800, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

12EMwj8FisScfsi.jpg

 IHC analysis of Phospho-GSK3 Beta(S9)(GB150050).
Sample: Human ovarian cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 800, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

13MogmfUKF9wIbF.jpg

 IHC analysis of Phospho-GSK3 Beta(S9)(GB150050).
Sample: Human stomach, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 800, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

14CnVmeBFzmq1PC.jpg

 IF analysis of Phospho-GSK3 Beta(S9)(GB150050)green.
Sample: Human renal carcinoma, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 200, 4℃ overnight.
Secondary antibody: 488 conjugated Goat Anti-Rabbit IgG (H+L) (GB25303), 1: 400, RT, 1h.

15DG6mPfxxlDqsU.jpg

 IF analysis of Phospho-GSK3 Beta(S9)(GB150050)green.
Sample: Human colon cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 200, 4℃ overnight.
Secondary antibody: 488 conjugated Goat Anti-Rabbit IgG (H+L) (GB25303), 1: 400, RT, 1h.

16ODuuUvDz08yce.jpg

 IF analysis of Phospho-GSK3 Beta(S9)(GB150050)green.
Sample: Human Hepatobiliary Cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 200, 4℃ overnight.
Secondary antibody: 488 conjugated Goat Anti-Rabbit IgG (H+L) (GB25303), 1: 400, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


Antibodies-Oncology
: AO-03-GB150050-50
: 10 Produits
Hurry! only 10 items left in stock.

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