Recombinant Anti-Phospho-STAT3(Y705) antibody (Rabbit mAb)

Protein full name

Signal transducer and activator of transcription 3

Synonyms

APRF, ADMIO, APRF, HIES, ADMIO1, Acute-phase response factor, STAT3, p-STAT3, p-stat3

Immunogen

KLH conjugated Synthetic phosphopeptide corresponding to Human STAT3(Tyr705)

Uniprot ID

P40763

Isotype

IgG, κ

Clone Number

SB99

Purity

Affinity purification

Subcellular location

Nucleus, Cytoplasm

Predicted MW. / Observed MW.

79/86 kDa / 79/86 kDa

Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1: 1000-1: 2000

C2CL2,HeLa

IHC/IF

Human, Mouse, Rat

1: 500-1: 2000

renal carcinoma , subcutaneous nude-mouse model of 4T1 cell

Storage

Storage

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Storage Buffer

PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

background

Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors.Once activated, recruits coactivators, such as NCOA1 or MED1, to the promoter region of the target gene.May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4.Upon activation of IL6ST/gp130 signaling by interleukin-6 (IL6), binds to the IL6-responsive elements identified in the promoters of various acute-phase protein genes.

Images

WB analysis of Phospho-STAT3(Y705)(GB150001).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

IHC analysis of Phospho-STAT3(Y705)(GB150001).
Sample: Human renal carcinoma, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

IHC analysis of Phospho-STAT3(Y705)(GB150001).
Sample: Mouse subcutaneous nude-mouse of 4T1 cell, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.