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Product Information
| Protein full name | Mitogen-activated protein kinase 8/9/10 |
| Synonyms | JNK1 + JNK2 + JNK3, JNK1/2/3, JNK1+2+3, MAPK8, MAPK9, MAPK10, c Jun N terminal kinase 1, c Jun N terminal kinase 2, c Jun N terminal kinase 3, P-JNK |
| Uniprot ID | P45983/P45984/P53779 |
| Immunogen | KLH conjugated Synthetic peptide corresponding to Human JNK(T183/Y185) |
| Isotype | IgG2b, κ |
| Clone Number | 8F8C2 |
| Predicted MW./Observed MW. | 46/54 kDa / 46/54 kDa |
| Purity | Affinity purification |
| Subcellular location | Cytoplasm, Nucleus |
Applications
| Applications | Species | Dilution | Positive Tissue |
| WB | Human | 1: 500-1: 1000 | HT-29 |
| IHC/IF | Human | 1: 500-1: 1000 | ovarian cancer, esophageal cancer, colon cancer |
Background
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase targets specific transcription factors, and thus mediates immediate-early gene expression in response to various cell stimuli. It is most closely related to MAPK8, both of which are involved in UV radiation induced apoptosis, thought to be related to the cytochrome c-mediated cell death pathway. This gene and MAPK8 are also known as c-Jun N-terminal kinases.
Images
| | WB analysis of Phospho-JNK (T183/Y185)(GB12018). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 5000, RT, 1h. |
| | IHC analysis of Phospho-JNK (T183/Y185)(GB12018). Sample: Human ovarian cancer, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h. |
| | IHC analysis of Phospho-JNK (T183/Y185)(GB12018). Sample: Human colon cancer, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h. |
| | IHC analysis of Phospho-JNK (T183/Y185)(GB12018). Sample: Human colon, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 2000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h. |
| | IHC analysis of Phospho-JNK (T183/Y185)(GB12018). Sample: Human colon, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h. |
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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