Anti-Phospho-CREB (S133) Rabbit pAb

Protein full name

Cyclic AMP-responsive element-binding protein 1

Synonyms

CREB-1, CAMP-responsive element-binding protein 1, CAMP responsive element binding protein 1, Active transcription factor CREB, P-CREB

Immunogen

KLH conjugated Synthetic phosphopeptide corresponding to Human CREB (S133).
The antibody can detect the phosphorylated form of CREB related protein ATF-1.

Uniprot ID

P16220

Isotype

IgG

Purity

Affinity purification

Subcellular location

Nucleus

Predicted MW./Observed MW.

35 kDa / 43 kDa

Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1: 500-1: 1000

293, PC-12, NIH/3T3

IHC/IF

Human, Mouse, Rat

1: 700-1: 1400

breast cancer, lung

Storage

Storage

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Storage Buffer

PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

background

Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells.

Images

WB analysis of Phospho-CREB (S133)(GB114322).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

WB analysis of Phospho-CREB (S133)(GB114322).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

IHC analysis of Phospho-CREB (S133)(GB114322).
Sample: Human Breast cancer non-phospho-blocking, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 700, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.