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Product Information
| Protein full name | Transcriptional coactivator YAP1 |
| Synonyms | 65 kDa Yes associated protein, YAP, YAP1, YAP2, YAP65, YKI, Yorkie homolog, P-YAP1 |
| Uniprot ID | P46937 |
| Immunogen | KLH conjugated Synthetic phosphopeptide corresponding to human YAP1(S127) |
| Isotype | IgG |
| Predicted MW./Observed MW. | 48-54 kDa / 78 kDa |
| Purity | Affinity purification |
Applications
| Applications | Species | Dilution | Positive Tissue |
| WB | Human, Mouse, Rat | 1: 300-1: 500 | SW480, HepG2, NIH/3T3 |
Background
Yes-associated protein 1 (YAP1) is a transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ.
Images
| | WB analysis of Phospho-YAP1(S127)(GB114060). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h. |
| | WB analysis of Phospho-YAP1(S127)(GB114060). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h. |
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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