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Product Information
| Protein full name | Microtubule-associated protein tau |
| Synonyms | Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau, MAPT, MAPTL, MTBT1, TAU, P-TAU |
| Uniprot ID | P10636-8 |
| Immunogen | KLH conjugated Synthetic phosphopeptide corresponding to Human TAU (S202/T205) |
| Isotype | IgG |
| Predicted MW./Observed MW. | 79 kDa / 50-80 kDa |
| Purity | Affinity purification |
| Subcellular location | Cytoplasm, Cytoskeleton, Secreted, Cell membrane, Cell projection |
Applications
| Applications | Species | Dilution | Positive Tissue |
| WB | Human, Mouse, Rat | 1: 500-1: 1000 | brain, hippocampus, liver |
| IHC/IF | Human, Mouse, Rat | 1: 600-1: 1200 | brain |
Background
Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity .The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both .Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
Images
| | WB analysis of Phospho-TAU (S202/T205)(GB113883). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h. |
| | WB analysis of Phospho-TAU (S202/T205)(GB113883). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h. |
| | IHC analysis of Phospho-TAU (S202/T205)(GB113883). Sample: Mouse AD brain, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min. Primary antibody: 1: 1200, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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